Aim. To identify the yeasts species isolated from urine and to determine the susceptibility to antifungal agents.

Material and method. A total number of 141 strains isolated between 1.01.2007- 30.06.2008 at National Institute of Infectious Diseases “ Matei Balş” – Bucharest (Romania) were identified with ID32C (bioMérieux, France). Antifungal susceptibility tests were made for 5-fluorocytosine, amphotericin B, fluconazole, itraconazole and voriconazole using ATB Fungus 2 şi 3. (bioMérieux, France). MIC to caspofungin was established by Etest for some of the strains.

Results. The number of urinary tract infections produced by fungi increased in the last months (86 strains isolated in 2007 and 55 in the first six months 2008). There were identified 55 strains C. albicans (39%), 22 C. glabrata (15,6%), 20 C. tropicalis (14,1%), 10 Trichosporon asahii (7,09%) and 9 C. kefyr (6,3%). The other strains were C. krusei (5), C.lusitaniae (4), C. inconspicua/norvegensis (4), C. valida (2), C. utilis (2), C. guillermondii, C. sake and C. parapsillosis, each with one strains. The majority of the strains were susceptible to 5-fluorocytosine and amphotericin B. Resistance to fluconazole varied between 27.2% and 30%, to itraconazole 31.8% - 70% and to voriconazole 15% - 40%. The most resistant species were C. glabrata, C.tropicalis and Trichosporon asahii.

Discussions. It is necessarily to establish the yeast species and to determine the susceptibility to antifungal agents for the optimal treatment of infection.