Aim: The performed research has consisted in mycotoxicological tests performed on dairy cows feed from individual farms.
Material and method: In 2004 we have processed a total number of 42 feed samples collected in diary farms from Dolj and Giurgiu counties. The mycotoxicological examination has been performed through immunoenzymatic assay (ELISA) for aflatoxins (AF), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEA) and T-2 toxin (T- 2).
Results: From hay samples collected in Giurgiu county, AF had an incidence of 0% (0 samples), OTA of 66.6% (4 samples), ZEA of 16.6% (one sample), DON of 50% (3 samples) and T-2 of 16.6% (one sample); in concentrated feed from the same county, the following incidence of mycotoxins has been occured: AF 53.3% (8 samples), OTA 73.3% (11 samples), ZEA 26.6% (4 samples), DON 53.3% (8 samples) and T-2 40% (6 samples). From the hay samples collected in Dolj county, AF occured in 0 samples (0%), OTA in 4 samples (66.6%), ZEA in 3 samples (50.0%), DON in 2 samples (33.3%) and T-2 in 2 samples (33.3%); in concentrated feed, the mycotoxins have been detected in the following percentages: AF 60.0% (9 samples), OTA 66.6% (10 samples), ZEA 26.6% (4 samples), DON 46.6% (7 samples) and T-2 40.0% (6 samples).
Discussion: The results have been statistically analyzed using the JMP 6.0 soft (SAS, 2005). The results regarding the concentration of mycotoxins in feed were judged according so the EC Regulations No. 1881/2006 and 1126/2007. In Dolj county, from a total number of 21 analyzed samples, the identified mycotoxins were: OTA 71.42% (15 samples), DON 52.38% (11 samples), T-2 33.33% (7 samples), AF 38.09% (8 samples) and ZEA 23.80% (5 samples). In Giurgiu county, from a total number of 21 analyzed samples, the identified mycotoxins were: OTA 66.6% (14 samples), AF, ZEA and DON 42.8% each (9 samples) and T-2 38.0% (8 samples).