Cyclodextrins are well known host molecules able to form inclusion complexes with a wide variety of guest molecules, including drugs (such as sulconazole nitrate - a very efficient, but also toxic antifungal drug). The effects of the addition of different cyclodextrins on the light absorption of sulconazole have been investigated in aqueous media. The formation of host-guest inclusion complexes was indicated by UV spectroscopy. The binding constants sulconazole to α-, β-, γ- and hydroxypropyl-β-cyclodextrin in bidistilled water have been determined Hildebrand-Benesi and Scatchard approximation. This method offers the possibility to investigate the association probability between cyclodextrins and Sulc, the strength of the binding forces and the future changes of drug behavior in aqueous medium induced by complexation process.